一、基本信息：

姓 名： 逄大欣

职 称：教授 博士生导师

所在部门：生物技术系

研究方向：转基因技术/基因表达与调控

办公电话：87835175

电子邮件：[email protected]

二、学习与工作经历

1.受教育经历

2003.09–2006.06六宝典资料生物化学与分子生物学 理学博士

2000.09–2003.06解放军军需大学生物化学与分子生物学理学硕士

1987.09–1991.06白求恩医科大学医学士

2.工作经历

2012.10–至今六宝典资料教授

2006.10–2012.09六宝典资料畜牧兽医学院副教授

1991.07–2006.09白求恩医科大学图书馆助教/讲师

三、主要教学工作（承担的本科生、研究生课程）

本科课程：基因工程、基因工程综合实验

硕士生课程：分子生物学新技术

四、主要科研工作（包括科研项目）

1.节粮型高瘦肉率转基因猪新品种培育--MSTN基因精确修饰猪的制备及培育（2016ZX08006001-004）转基因生物新品种培育重大专项287.98万元分题主持人在研

2.节粮型高瘦肉率转基因猪新品种培育--MSTN基因敲除猪新品种培育（2011ZX08006003）转基因生物新品种培育重大专项330.02万元分题主持人已结题

3.节粮型高瘦肉率转基因猪新品种培育--转MSTN基因猪培育（2008ZX08006003）转基因生物新品种培育重大专项288.00万元分题主持人已结题

4.肌生成抑制素与对其活性有影响的几 种因子相互作用研究（30871841）国家自然科学基金小额资助项目8.00万元主持人已结题

5.猪肌生成抑制素受体的筛选（30771582）国家自然科学基金面上项目28万元主要参加已结题

6.猪肌生成抑制素作用靶位点的研究（300700563）国家自然科学基金面上项目20万元主要参加已结题

五、科研成果（论文）

1、Li M, Ouyang H, Yuan H, Li J, Xie Z, Wang K, Yu T, Liu M, Chen X, Tang X, JiaoH, Pang D. Site-Specific Fat-1 Knock-In Enables Significant Decrease of n-6PUFAs/n-3PUFAs Ratio in Pigs. G3 (Bethesda). 2018;8(5):1747-1754.通讯作者2.742

2、Wang K, Tang X, Xie Z, Zou X, Li M, Yuan H, Guo N, Ouyang H, Jiao H, Pang D.CRISPR/Cas9-mediated knockout of myostatin in Chinese indigenous Erhualian pigs. Transgenic Res. 2017;26(6):799-805.通讯作者2.197

3、Xie Z, Pang D, Wang K, Li M, Guo N, Yuan H, Li J, Zou X, Jiao H, Ouyang H, Li Z, Tang X. Optimization of a CRISPR/Cas9-mediated Knock-in Strategy at the Porcine Rosa26 Locus in Porcine Foetal Fibroblasts. Sci Rep. 2017;7(1):3036.并列作者4.122

4、Wang K, Tang X, Liu Y, Xie Z, Zou X, Li M, Yuan H, Ouyang H, Jiao H, Pang D. Efficient Generation of Orthologous Point Mutations in Pigs via CRISPR-assisted ssODN-mediated Homology-directed Repair. Mol Ther Nucleic Acids. 2016;5(11):e396.通讯作者5.660

5、Peng Z, Ouyang T, Pang D, Ma T, Chen X, Guo N, Chen F, Yuan L, Ouyang H, Ren L. Pseudorabies virus can escape from CRISPR-Cas9-mediated inhibition. Virus Res.2016;223:197-205.并列作者2.484

6、Huang Y, Li Z, Wang A, Han X, Song Y, Yuan L, Li T, Wang B, Lai L, Ouyang H, Pang D. Chimerism in piglets developed from aggregated cloned embryos. FEBS Open Bio. 2016;6(4):285-302.通讯作者1.782

7、Liu X, Pang D, Yuan T, Li Z, Li Z, Zhang M, Ren W, Ouyang H, Tang X. N-3 polyunsaturated fatty acids attenuates triglyceride and inflammatory factors level in hfat-1 transgenic pigs. Lipids Health Dis. 2016;15:89.并列作者2.663

8、Peng Z, Ma T, Pang D, Su D, Chen F, Chen X, Guo N, Ouyang T, Ouyang H, Ren L. Expression, purification and antibody preparation of PCV2 Rep and ORF3 proteins. Int J Biol Macromol. 2016;86:277-81.并列作者3.909

9、Wang K, Ouyang H, Xie Z, Yao C, Guo N, Li M, Jiao H, Pang D. Efficient Generation of Myostatin Mutations in Pigs Using the CRISPR/Cas9 System. Sci Rep. 2015;5:16623.通讯作者4.122

10、Tang X, Wang G, Liu X, Han X, Li Z, Ran G, Li Z, Song Q, Ji Y, Wang H, Wang Y, Ouyang H, Pang D. Overexpression of porcine lipoprotein-associated phospholipase A2 in swine. Biochem Biophys Res Commun. 2015 Sep 25;465(3):507-11.通讯作者4.122

11、Liu S, Li J, Yuan T, Yang F, Pang D, Tu C. [Quantitative detection of anti-classical swine fever virus siRNA expression in cells by stem-loop RT-qPCR].Sheng Wu Gong Cheng Xue Bao. 2012 Jan;28(1):26-36.通讯作者0.19

12、Zhu J, Pang D, Zhou Y, Tang X, Huang Y, Xie W, Gao F, Lai L, Zhang M, Ouyang H. Direct conversion of porcine embryonic fibroblasts into adipocytes by chemical molecules. Cell Reprogram. 2012;14(2):99-105.并列作者1.430

16、Huang Y, Tang X, Xie W, Zhou Y, Li D, Yao C, Zhou Y, Zhu J, Lai L, Ouyang H, Pang D. Histone deacetylase inhibitor significantly improved the cloning efficiency of porcine somatic cell nuclear transfer embryos. Cell Reprogram. 2011 Dec;13(6):513-20.通讯作者1.430

17、Huang Y, Tang X, Xie W, Zhou Y, Li D, Zhou Y, Zhu J, Yuan T, Lai L, Pang D,Ouyang H. Vitamin C enhances in vitro and in vivo development of porcine somatic cell nuclear transfer embryos. Biochem Biophys Res Commun. 2011;411(2):397-401.通讯作者2.559

18、Tan G, Ren L, Huang Y, Tang X, Zhou Y, Zhou Y, Li D, Song H, Ouyang H, Pang D. Isolation and culture of embryonic stem-like cells from pig nuclear transfer blastocysts of different days. Zygote. 2012;20(4):347-52通讯作者1.114

19、Zhou Y, Ren L, Zhu J, Yan S, Wang H, Song N, Li L, Ouyang H, Pang D.Construction of a recombinant human FGF1 expression vector for mammary gland-specific expression in human breast cancer cells. Mol Cell Biochem. 2011;354(1-2):39-46.通讯作者2.561

20、Zhao Y, Pang D, Wang T, Yang X, Wu R, Ren L, Yuan T, Huang Y, Ouyang H. Human MxA protein inhibits the replication of classical swine fever virus. Virus Res.2011;156(1-2):151-5.并列作者2.484

21、Li L, Pang D, Chen L, Wang T, Nie D, Yan S, Ouyang H. Establishment of a transgenic pig fetal fibroblast reporter cell line for monitoring Cre recombinase activity. DNA Cell Biol. 2009;28(6):303-8.并列作者2.484并列作者2.634

22、Li L, Pang D, Wang T, Li Z, Chen L, Zhang M, Song N, Nie D, Chen Z, Lai L,Ouyang H. Production of a reporter transgenic pig for monitoring Cre recombinase activity. Biochem Biophys Res Commun. 2009;382(2):232-5.并列作者2.559