一、基本信息：

姓 名：袁宝

职 称：博士，教授，博士研究生导师

研究方向：动物繁殖调控，家畜实验动物标准化

办公电话：0431-87836536

电子邮件：[email protected]，[email protected]

二、学习与工作经历：

2019.10 ~今：六宝典资料，六宝典资料，教授

2015.10 ~ 2019.09：六宝典资料，六宝典资料，副教授

2015.08 ~ 2017.01：韩国忠北国立大学，农业与生命学院，访问学者

2008.10 ~ 2015.09：六宝典资料，六宝典资料，讲师

2004.07 ~ 2008.09：六宝典资料，实验动物中心，助教

三、主要教学工作（承担的本科生、研究生课程）

主要承担本科生及研究生《动物繁殖生物技术》、《动物生产学实习》，《实验动物方法学》,《比较医学概论》,等课程的教学任务。

四、主要科研项目

1. 国家肉牛牦牛产业技术体系-良种扩繁与生产技术岗位，2022.01-2025.12，经费238万元。

2. 国家重点研发专项，科右前旗优势特色产业关键技术集成与示范，2022.10-2025.03，经费30万元。

3. 国家自然基金面上项目，circAkap17b通过MAPK信号通路调控牛腺垂体FSH分泌机制研究（32172729），2022.01-2025.12，经费60万元。

4. 国家自然基金面上项目，lncRNA-M7s1—miR-7通过Raf/MEK/ERK信号通路调控牛腺垂体FSH分泌机制的研究（31872349），2019.01-2022.12，经费60万元。

5. 肉牛种群繁育及健康养殖关键技术熟化与示范，2023.03-2024.12，经费45万元。

6. 国家重点研发专项，实验羊的开发、评价与应用（2022YFF0710504），2022.11-2026.10，经费320万元。

7. 动物微生态制剂生产技术的研发，2023.03-2025.12，经费100万元。

8. 省直厅局项目，实验牛微生物等级及监测，2022.09-2023.09，经费5万元。

9. 吉林省科技厅基础处项目，实验动物吉林省重点实验室（YDZJ202102CXJD019），2021.01-2023.12，经费40万元。

10. 吉林省科技厅计划处项目，吉林省实验动物领域地方标准制定体系建立（20200703006ZP），2020.01-2022.12，经费10万元。

11. 吉林省科技厅基础处项目，lncRNA-M7s2通过Raf信号通路调控牛腺垂体FSH分泌的ceRNA机制的研究（20190201166JC），2019.01-2021.12，经费10万元。

12. 利用GGP基因芯片对延边黄牛核心群评分，2019.01-2020.12，经费4万元。

五、主要科研成果（著作、专利、论文）

1. Ji ZH, Gao F, Xie WY, Wu HY, Ren WZ, Yuan B（通讯）. Mammary Epithelial Cell-Derived Exosomal miR-221-3p Regulates Macrophage Polarization by Targeting Igf2bp2 during Mastitis. J Agric Food Chem. 2023 Oct 11;71(40):14742-14757.

2. Liu ZB, Zhang JB, Li SP, Yu WJ, Pei N, Jia HT, Li Z, Lv WF, Wang J, Kim NH, Yuan B（通讯）, Jiang H. ID3 regulates progesterone synthesis in bovine cumulus cells through modulation of mitochondrial function. Theriogenology. 2023 Oct 1;209:141-150.

3. Wang Y, Qi JJ, Yin YJ, Jiang H, Zhang JB, Liang S, Yuan B（通讯）. Ferulic Acid Enhances Oocyte Maturation and the Subsequent Development of Bovine Oocytes. Int J Mol Sci. 2023 Sep 30;24(19):14804.

4. Lyu C, Yuan B（共一）, Meng Y, Cong S, Che H, Ji X, Wang H, Chen C, Li X, Jiang H, Zhang J. Puerarin Alleviates H2O2-Induced Oxidative Stress and Blood-Milk Barrier Impairment in Dairy Cows. Int J Mol Sci. 2023 Apr 24;24(9):7742.

5. Zheng Y, Zhou Y, Huang Y, Wang H, Guo H, Yuan B（通讯）, Zhang J. Transcriptome sequencing of black and white hair follicles in the giant panda. Integr Zool. 2023 May;18(3):552-568.

6. Wang T, Zhao G, Yu S, Zheng Y, Guo H, Wang H, Zhao P, Xie W, Ren W, Yuan B（通讯）. Sequencing of the Pituitary Transcriptome after GnRH Treatment Uncovers the Involvement of lncRNA-m23b/miR-23b-3p/CAMK2D in FSH Synthesis and Secretion. Genes (Basel). 2023 Mar 31;14(4):846.

7. Fu Y, Zhang JB, Han DX, Wang HQ, Liu JB, Xiao Y, Jiang H, Gao Y, Yuan B（通讯）. CiRS-187 regulates BMPR2 expression by targeting miR-187 in bovine cumulus cells treated with BMP15 and GDF9. Theriogenology. 2023 Feb;197:62-70.

8. Che HY, Zhou CH, Lyu CC, Meng Y, He YT, Wang HQ, Wu HY, Zhang JB, Yuan B（通讯）. Allicin Alleviated LPS-Induced Mastitis via the TLR4/NF-κB Signaling Pathway in Bovine Mammary Epithelial Cells. Int J Mol Sci. 2023 Feb 14;24(4):3805.

9. Liu JB, Zhang JB, Yan XM, Xie PG, Fu Y, Fu XH, Sun XL, Han DX, Li SP, Zheng Y, Gao Y, Kim NH, Yuan B（通讯）, Jiang H. DNA Double-Strand Break-Related Competitive Endogenous RNA Network of Noncoding RNA in Bovine Cumulus Cells. Genes (Basel). 2023 Jan 22;14(2):290.

10. Zhao GK, Zheng Y, Guo HX, Wang HQ, Ji ZH, Wang T, Yu S, Zhang JB, Yuan B（通讯）, Ren WZ. TRH Regulates the Synthesis and Secretion of Prolactin in Rats with Adenohypophysis through the Differential Expression of miR-126a-5p. Int J Mol Sci. 2022 Dec 14;23(24):15914.

11. Guo HX, Zheng Y, Zhao GK, Wang HQ, Yu S, Gao F, Zhang JB, Zhang YH, Yuan B（通讯）. Circ-ERC2 Is Involved in Melatonin Synthesis by Regulating the miR-125a-5p/MAT2A Axis. Int J Mol Sci. 2022 Dec 7;23(24):15477.

12. Yu W, Jiang H, Liu F, Li Z, Xu L, Liu C, Lv W, Wang J, Gao Y, Liang S, Kim NH, Zhang J, Chen C, Yuan B（通讯）. KRAS Affects the Lipid Composition by Regulating Mitochondrial Functions and MAPK Activation in Bovine Mammary Epithelial Cells. Animals (Basel). 2022 Nov 8;12(22):3070.

13. Ji ZH, Ren WZ, Wu HY, Zhang JB, Yuan B（通讯）. Exosomes in Mastitis-Research Status, Opportunities, and Challenges. Animals (Basel). 2022 Oct 21;12(20):2881.

14. Ji ZH, Ren WZ, Wang HQ, Gao W, Yuan B（通讯）. Molecular Subtyping Based on Cuproptosis-Related Genes and Characterization of Tumor Microenvironment Infiltration in Kidney Renal Clear Cell Carcinoma. Front Oncol. 2022 Jul 6;12:919083.

15. Wang HQ, Zhang JB, Zheng Y, Zhang WD, Guo HX, Cong S, Ding Y, Yuan B（通讯）. Comprehensive analysis of differences in N6-methyladenosine RNA methylomes in the rat adenohypophysis after GnRH treatment. FASEB J. 2022 Mar;36(3):e22204.

16. Zheng Y, Wang HQ, Guo HX, Xie HL, Zhang WD, Han DX, Jiang H, Yuan B（通讯）, Zhang JB. CircRNA-WNK2 Acts as a ceRNA for miR-328a-3p to Promote AANAT Expression in the Male Rat Pineal Gland. Endocrinology. 2022 Feb 1;163(2):bqab255.

17. Zhang W, Ren W, Han D, Zhao G, Wang H, Guo H, Zheng Y, Ji Z, Gao W, Yuan B（通讯）. LncRNA-m18as1 competitively binds with miR-18a-5p to regulate follicle-stimulating hormone secretion through the Smad2/3 pathway in rat primary pituitary cells. J Zhejiang Univ Sci B. 2022 Jun 15;23(6):502-514.

18. Wang HQ, Wang WH, Chen CZ, Guo HX, Jiang H, Yuan B（通讯）, Zhang JB. Regulation of FSH Synthesis by Differentially Expressed miR-488 in Anterior Adenohypophyseal Cells. Animals (Basel). 2021 Nov 15;11(11):3262.

19. Guo HX, Yuan B（共一）, Su MT, Zheng Y, Zhang JY, Han DX, Wang HQ, Huang YJ, Jiang H, Zhang JB. Identification of Circular RNAs in the Anterior Pituitary in Rats Treated with GnRH. Animals (Basel). 2021 Aug 31;11(9):2557.

20. Xiao Y, Yuan B（共一）, Hu W, Qi J, Jiang H, Sun B, Zhang J, Liang S. Tributyltin Oxide Exposure During in vitro Maturation Disrupts Oocyte Maturation and Subsequent Embryonic Developmental Competence in Pigs. Front Cell Dev Biol. 2021 Jun 28;9:683448.

21. Wang HQ, Zhang WD, Yuan B（通讯）, Zhang JB. Advances in the Regulation of Mammalian Follicle-Stimulating Hormone Secretion. Animals (Basel). 2021 Apr 15;11(4):1134.

22. Huang YJ, Gao Y, Wang CJ, Han DX, Zheng Y, Wang WH, Jiang H, Yuan B（通讯）, Zhang JB. Hydroxyurea regulates the development and survival of B16 Melanoma Cells by upregulating MiR-7013-3p. Int J Med Sci. 2021 Mar 3;18(8):1877-1885.

23. Yu Z, Ren W, Wang T, Zhang W, Wang C, Wang H, Gao F, Yuan B（通讯）. CircAgtpbp1 Acts as a Molecular Sponge of miR-543-5p to Regulate the Secretion of GH in Rat Pituitary Cells. Animals (Basel). 2021 Feb 20;11(2):558.

24. Zheng Y, Jiang H, Wang HQ, Guo HX, Han DX, Huang YJ, Gao Y, Yuan B（通讯）, Zhang JB. Identifying daily changes in circRNAs and circRNA-associated-ceRNA networks in the rat pineal gland. Int J Med Sci. 2021 Jan 14;18(5):1225-1239.

25. Wang MQ, Zhou CH, Cong S, Han DX, Wang CJ, Tian Y, Zhang JB, Jiang H, Yuan B（通讯）. Lipopolysaccharide inhibits triglyceride synthesis in dairy cow mammary epithelial cells by upregulating miR-27a-3p, which targets the PPARG gene. J Dairy Sci. 2021 Jan;104(1):989-1001.

26. Han DX, Sun XL, Wang CJ, Yu ZW, Zheng Y, Huang YJ, Wang WH, Jiang H, Gao Y,Yuan B（通讯）, Zhang JB. Differentially expressed lncRNA-m433s1 regulates FSH secretion by functioning as a miRNA sponge in male rat anterior pituitary cellsdagger. Biol Reprod 2019, 101(2): 416-425.

27. D.X. Han, C.J. Wang, X.L. Sun, J.B. Liu, H. Jiang, Y. Gao, C.Z. Chen,Yuan B（通讯）, J.B. Zhang, Identification of circular RNAs in the immature and mature rat anterior pituitary, J Endocrinol, 240 (2019) 393-402.

28. Zhang Z, Wang W, Liu JB, Wang Y, Hao JD, Huang YJ, Gao Y, Jiang H,Yuan B（通讯）, Zhang JB. ssc-miR-204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2. J Cell Biochem 2019.

29. C.J. Wang, H.X. Guo, D.X. Han, Z.W. Yu, Y. Zheng, H. Jiang, Y. Gao,Yuan B（通讯）, J.B. Zhang, Pituitary tissue-specific miR-7a-5p regulates FSH expression in rat anterior adenohypophyseal cells, PeerJ, 7 (2019) e6458.

30. Yu ZW, Gao W, Feng XY, Zhang JY, Guo HX, Wang CJ, Chen J, Hu JP, Ren WZ,Yuan B（通讯）. Roles of differential expression of miR-543-5p in GH regulation in rat anterior pituitary cells and GH3 cells. PLoS One 2019, 14(9): e0222340.

31. Zhang Z, Chen CZ, Xu MQ, Zhang LQ, Liu JB, Gao Y, Jiang H, Yuan B（通讯）, Zhang JB. MiR-31 and miR-143 affect steroid hormone synthesis and inhibit cell apoptosis in bovine granulosa cells through FSHR. Theriogenology. 2019 Jan 1;123:45-53.

32. 一种验证miRNA对于大鼠腺垂体GH分泌调控作用的方法，发明，中国，ZL201811585690.6，2022.04

33. 一种预测弱畸精子症体外受精能力的方法，发明，中国，ZL201810001267.0，2021.10

34. ATG10基因作为牛超数排卵性状分子标记的应用，发明，中国，ZL201811390985.8，2022.04

六、奖励与荣誉

1. 非编码RNA调控动物腺垂体促卵泡素合成与分泌的机制，吉林省自然科学奖，二等奖（1/12），吉林省科学技术厅

2. 优质肉牛良种扩繁与生产技术体系的建立与应用，吉林省科学技术进步奖，二等奖（2/12），2019.11，吉林省科学技术厅。

3. 优质肉牛规范化生产技术体系研究与集成示范，吉林省科学技术进步奖，一等奖（10/15），2012.09，吉林省科学技术厅。